Pooled library tissue tags for EST-based gene discovery
نویسندگان
چکیده
منابع مشابه
Development of New Placental and Fetal Expressed Sequence Tags (EST) for Gene Discovery in Pig Reproduction
One major problem that has high economic impact on pig reproduction is the unexplained loss of potential porcine conceptuses during the first month of gestation. To better understand when and how these losses occur, it is imperative to investigate the underlying genetic regulatory mechanisms. We have recently initiated a large-scale cDNA sequencing project to provide molecular information regar...
متن کاملP-215: Discovery of A Novel APA Variant of A Human Potential Gene Based on Expressed Sequenced Tags Analysis
Background: Expressed sequence tags (ESTs) are sequences of cDNA fragments prepared from different tissue sources. There are over one million of these sequences in the publicly available database, and these sequences are believed to represent more than half of all human genes. The ESTs belong to different cDNA libraries, was prepared from one particular cell type, organ, or tumor. Therefore, th...
متن کاملInformatics for Efficient Est-based Gene Discovery in Normalized and Subtracted Cdna Libraries
Beginning in 1997, large-scale efforts in EST-based gene discovery in Rat, Human, Mouse, and other species have been conducted at the University of Iowa. To date, these efforts have led to the sequencing of more than 400,000 ESTs accounting for more than 60,000 novel ESTs, including 40,000 previously undescribed genes. This effort has been augmented by a set of custom informatics tools to gathe...
متن کاملInformatics for Efficient EST-Based Gene Discovery in Normalized and Subtracted cDNA Libraries
Structured Abstract Motivation: Large-scale Gene Discovery projects based on EST sequencing are important for providing catalogs of cDNAs for various purposes. To provide accurate estimates of gene discovery, the methods used must be robust in the presence of sequence errors. Efficient informatics systems play a vital role in a number of aspects of the process: serial subtraction, clustering, a...
متن کاملGene Identification and Expression Analysis of 86,136 Expressed Sequence Tags (EST) from the Rice Genome
Expressed Sequence Tag (EST) analysis has pioneered genome-wide gene discovery and expression profiling. In order to establish a gene expression index in the rice cultivar indica, we sequenced and analyzed 86,136 ESTs from nine rice cDNA libraries from the super hybrid cultivar LYP9 and its parental cultivars. We assembled these ESTs into 13,232 contigs and leave 8,976 singletons. Overall, 7,49...
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ژورنال
عنوان ژورنال: Bioinformatics
سال: 2002
ISSN: 1367-4803,1460-2059
DOI: 10.1093/bioinformatics/18.9.1162